Ole fa'asusu su'esu'e ose fa'atupuina ile fa'aputuga vai.O loʻo i ai le lelei o le faʻasologa o le gaosiga o faʻalavelave, faʻasolosolo tausaga faʻamaʻi, inoculation faigofie, talafeagai mo le gaosiga o fale gaosimea, ma isi.Ua fa'afeiloa'ia e le to'atele o faifa'ato'aga.Lu'u sela (https://www.luoron.com/plastic-erlenmeyer-flask-with-vent-cap-2-product/） o se ituaiga o mea faʻaaogaina e manaʻomia i le aganuu o faʻamaʻi vai.O mea nei e mana'omia ona va'aia ile aganu'u:

1. Sauniuniga o aganuu masani e tusa ai ma le fua o vaega eseese, i totonu o le atigipusa, solo i vai, vaevaeina i fagu tafatolu, masani 250 ~ 300mL sela lulu fagu 50mL aganuu auala,500mL fagu tafatolu  (https://www.luoron.com/plastic-erlenmeyer-flask-with-vent-cap-2-product/）100mL ala fa'aleaganu'u, fa'apipi'i so'oga pulu, fa'atasi ai ma nusipepa afifi;E mafai foi ona faia i le 8 layers o gauze 8cm × 8cm fagu fa'amaufa'ailoga, fafo ma fa'amaufa'ailoga lanu enaena.
2. Autoclave sterilization auala e masani ona faaaogaina mo sterilization, lea e manaomia 121 ℃ ma 30min.Aveese i fafo ma fa'alili i le tusa ma le 30 ℃.Tuu i totonu o le potu mama po'o le pusa tui mo le fa'aoga.
3. E tusa ai ma manaʻoga faʻaogaina o le aseptic, e mafai ona faʻafesoʻotaʻi fagu taʻitasi ma le 2 ~ 3cm2 slant strain, ma e mafai ona faʻafesoʻotaʻi faʻamaʻi taʻitasi ma 4 ~ 5 fagu.E sili atu le aumaia o se mea faʻapipiʻi faʻatasi ai ma faʻalavelave faʻaopoopo, ina ia mafai ona faʻafefe i luga o le mea faʻafefe.A uma ona tui, ufiufi le gutu o le fagu i le kasa na fa'apipi'i muamua, ma nonoa mau i le manoa.
4. E mafai ona tuʻuina le fagu faʻafefeteina i totonu o se moega lulu mo aganuu, pe mafai ona tuʻuina i se vevela faifaipea o le 24 ~ 26 ℃ mo le 48h, ona mafai lea ona faʻataunuʻuina le aganuu pe a uma ona faʻalauteleina le mycelium aerogenic i totonu o le aganuu. .O le oscillating taimi ole lulu reciprocating e 80 ~ 120r/min, ma o le lulu rotary e 150 ~ 220r/min.3 ~ 4d e mafai ona faʻatupuina i le lulu.I le fa'ai'uga o le aganu'u, ona o fa'alavelave 'ese'ese, o le aganu'u fa'aleaganu'u na fa'aalia lanu eseese, e pei o le oyster mushroom, enoki mushroom culture medium sa lanu samasama;Mushroom, ericius erinaceus culture medium e lanu mumu enaena, ma le manogi mushroom;E lanu enaena, mafiafia ma suamalie le fuala'au aganu'u.E mafai ona faʻaaogaina le aganuʻu mo le gaosiga poʻo le faʻalauteleina o aganuʻu pe a oʻo i le 10g / L le mamafa mamago o le mycelium ma le lautele o pellets mycelium e 1 ~ 2mm.
 
5. Fa'agata fa'asusu lona lua gaosiga fa'aleaganu'u fa'aleaganu'u fa'atupuina o le tulaga tutusa o le vai, e tatau ona tele le pusa fa'aleaganu'u, e le sili atu i le 3500mL le gafatia.A mae'a ona fa'amama ma fa'alili, ona fa'aofi lea i totonu o vai vai fa'afefete5L sela lulu(https://www.luoron.com/3l5l-high-efficiency-erlenmeyer-flask-product/）i le fua faatatau o le 5%-10% ma fa'atupuina i luga o se lulu i se saoasaoa lemu.A maeʻa se taimi faʻapitoa o aganuʻu faʻafefe, e mafai ona maua le vai faʻafefe ma le tufatufaina tutusa o microspheres ma le vai faʻafefete manino.
I se aotelega, pe a faʻaaogaina le cell shaker e faʻatupuina ai faʻamaʻi vai, galue e tusa ai ma metotia o loʻo i luga, ma faʻalogo lelei e pulea le vevela, lulu taimi ma isi mea i le faagasologa e faʻamautinoa ai le aʻafiaga o aganuʻu.
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